Ghost-Sniper Column Chromasir HPLC UPLC ndime imachotsa nsonga za mizukwa

Ghost-Sniper Column Chromasir HPLC UPLC ndime kuchotsa nsonga za ghost

Kufotokozera mwachidule:

Mzere wa Ghost-Sniper ndi chida champhamvu chothetsera nsonga zamzukwa zomwe zimapangidwa panthawi yopatukana ndi chromatographic, makamaka mumayendedwe a gradient.Kukwera kwa mizukwa kungayambitse mavuto ochulukira ngati nsonga za mizukwa zidutsa nsonga zachidwi.Ndi gawo la Chromasir ghost-sniper, zovuta zonse zobwera chifukwa cha nsonga za ghost zitha kuthetsedwa ndipo mtengo woyesera ukhoza kutsika kwambiri.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Mzere wa Ghost-sniper umapangidwa makamaka kuti uthetse nsonga za mizukwa.Nsonga za mizimu ndizosadziwika bwino mu chromatogram, zomwe nthawi zambiri zimawonedwa panthawi ya kupatukana kwa chromatographic mu gradient elution kapena kugwira ntchito kwa nthawi yayitali.Kupezeka kwa nsonga za mizimu kumakhudza kwambiri zoyeserera za akatswiri.Mzere wa Ghost-sniper umachitika kuti uthetse mavutowa ndikupanga kuyesa kukhala kosavuta komanso kothandiza.Mzerewu ukhoza kugwiritsidwa ntchito mumkhalidwe wovuta kwambiri ndipo umasonyeza kukopa kwakukulu.Ndi njira yabwino yochotsera kusokonezedwa ndi nsonga za mizimu pakutsimikizira njira ndikutsata kusanthula kwazinthu.

Ma parameters

Gawo No.	Dimension	Voliyumu	Kugwiritsa ntchito
Chithunzi cha MC5046091P	50 × 4.6 mm	pa 800ul	Mtengo wa HPLC
Chithunzi cha MC3546092P	35 × 4.6 mm	pa 580l	Mtengo wa HPLC
Chithunzi cha MC5021093P	50 × 2.1 mm	pa 170l	UPLC
Chithunzi cha MC3040096P	30 × 4.0 mm	pa 380l	Mtengo wotsika kwambiri wa magawo a HPLC

Kuyika

Kugwiritsa Ntchito ndi Zotsatira

Kusamalitsa

1. Ngati kusanthula kwa batch kumakonzedwa mu dongosolo la HPLC, tikulimbikitsidwa kuti muwonjezere nthawi yowonjezera pafupifupi 5 min - 10 min mu chromatographic mikhalidwe yanu, chifukwa cha kuchuluka kwa gawo la ghost-sniper.
2. Kwa mizati yatsopano, yambani ndi 100% acetonitrile pa mlingo wothamanga wa 0.5ml / min kwa maola 4 musanagwiritse ntchito.
3. Ma ion-pair reagents mu gawo la mafoni, amatha kutengeka ndi gawo la ghost-sniper, zomwe zitha kukhudza nthawi yosungira komanso mawonekedwe apamwamba azomwe mukufuna.Chonde gwiritsani ntchito mosamala pansi pa gawo la mafoni.
4. Mzati wamoyo wonse umadalira mikhalidwe yowunikira, monga gawo la mafoni.kuyeretsedwa kwa zosungunulira, ndi kuipitsidwa kwa zida.Chonde sinthani gawo la ghost-sniper pafupipafupi kuti muwonetsetse kuti zikuyenda bwino.
5. Kusintha gawo la ghost-sniper kumaperekedwa ngati kugwidwa kukukulirakulira kapena zofunidwa sizingakwaniritsidwe.
6. Monga gawo loyeretsera ku chromatograph yamadzimadzi, ndime ya ghost-sniper imatha kusefa tinthu tating'onoting'ono ndikuchotsa zowononga organic pamaso pa jekeseni.Mzere wa ghost-sniper umaperekanso zida ndi gawo la chromatographic zotetezedwa bwino, komanso zimathandizira chromatogram.
7. Ngati gawo loyenda lili ndi mchere wotchingira, musanagwiritse ntchito komanso mukatha, tumizani ku 10% organic phase solution (10% methanol kapena acetonitrile), kuti mchere usatuluke ndikutchinga chigawocho.
8. Chonde dziwani kuti si nsonga zonse za mizimu zomwe zitha kugwidwa ndi gawo la Ghost-Sniper.
9. Ngati ndimeyi isiya kugwiritsidwa ntchito kwa nthawi yayitali, tikulimbikitsidwa kuti ikhale mu organic aqueous solution (70% methanol kapena acetonitrile).Ndipo chonde tsitsani ndi 100% acetonitrile pakuyenda kwa 0.5 ml / min kwa ola limodzi musanagwiritse ntchito.