Momwe Mungakulitsire Moyo Wanu wa Chromatography Column

Mu high-performance liquid chromatography (HPLC), zigawo zochepa ndizofunika kwambiri-kapena zokwera mtengo-monga chromatography column. Koma kodi mumadziwa kuti ndi chisamaliro choyenera ndi kusamalira, mutha kukulitsa kwambirichromatography column moyo wautalindikusintha labu yanu kuti igwire bwino ntchito?

Bukuli likuwunika maupangiri otsimikizirika okonzekera ndi njira zothandiza zomwe zingakuthandizeni kuteteza ndalama zanu ndikuwonetsetsa kuti zowunikira zimayendera pakapita nthawi.

Sankhani Gawo Loyenera Lam'manja kuyambira poyambira

Ulendo wopita kutalichromatography column moyo wautaliimayamba ndi kusankha mwanzeru zosungunulira. Gawo lolakwika la mafoni limatha kusokoneza zinthu zolongedza, kuchepetsa kusamvana, kapenanso kuwononga zomwe sizingasinthe. Nthawi zonse onetsetsani kuti pH, mphamvu ya ionic, ndi mtundu wa zosungunulira zimagwirizana ndi chemistry yanu.

Kuchotsa zosungunulira ndi kuzisefa musanagwiritse ntchito ndi njira zofunika kwambiri. Njira zodzitetezera izi zimalepheretsa kutsekeka kwa tinthu tating'ono komanso kupangika kwa kuwira kwa gasi, zonse zomwe zitha kusokoneza magwiridwe antchito.

Konzani Njira Yanu Yoyikira

Zomwe zimalowa m'gawoli zimangotengera momwe zimafikira pamenepo. Zitsanzo zochulukira kapena zomwe zili ndi tinthu tating'onoting'ono zimatha kufupikitsa moyo wogwiritsiridwa ntchito wa gawolo. Gwiritsani ntchito zitsanzo zokonzedwa bwino—zosefedwa kudzera muzosefera za 0.22 kapena 0.45 µm—kuteteza kutsekeka ndi kuwonjezereka kwamphamvu.

Ngati mukugwira ntchito ndi matrices ovuta kapena auve, ganizirani kugwiritsa ntchito khola la alonda kapena fyuluta ya pre-column. Zida zotsika mtengozi zimatha kutsekereza zoyipitsidwa zisanafike pagawo lowunikira, kukulitsa kwambirichromatography column moyo wautali.

Khazikitsani Njira Yoyeretsera Nthawi Zonse

Monga chida chilichonse cholondola, gawo lanu limafunikira kuyeretsedwa pafupipafupi kuti mugwire bwino ntchito. Kuchita bwino ndikutsuka ndimeyo mukatha kugwiritsa ntchito ndi chosungunulira chogwirizana, makamaka mukasinthana pakati pa makina a buffer kapena mitundu yachitsanzo.

Kuyeretsa mozama nthawi ndi nthawi ndi zosungunulira zamphamvu kumatha kuchotsa zinyalala zomwe zasonkhanitsidwa ndi mankhwala a hydrophobic. Onetsetsani kuti mukutsatira ndondomeko yoyeretsera mzati ndikupewa kugwiritsa ntchito mankhwala omwe angawononge malo osasunthika.

Sungani Pakati pa Kuthamanga

Kusungirako koyenera nthawi zambiri kumanyalanyazidwa, komabe kumachita gawo lalikulu pakusunga kwanuchromatography column moyo wautali. Ngati chigawo sichidzagwiritsidwa ntchito kwa nthawi yaitali, chiyenera kutsukidwa ndi chosungunulira choyenera - nthawi zambiri chimakhala ndi organic component kuteteza tizilombo toyambitsa matenda.

Nthawi zonse tsekani mbali zonse ziwiri mwamphamvu kuti musawume kapena kuipitsidwa. Kuti musunge nthawi yayitali, sungani mzatiwo pamalo oyera, otetezedwa ndi kutentha, kutali ndi kuwala kwachindunji ndi kutentha.

Yang'anirani Magwiridwe a Mgawo Nthawi Zonse

Kusunga chipika chakumbuyo chakumbuyo, nthawi yosungira, ndi mawonekedwe apamwamba kungakuthandizeni kuzindikira zizindikiro zoyambirira za kuwonongeka kwa mzere. Kusintha kwadzidzidzi pazigawo izi kumatha kuwonetsa kuipitsidwa, kutayika, kapena kutsekeka kwa frit.

Pozindikira izi msanga, mutha kuchitapo kanthu kukonza - monga kuyeretsa kapena kusintha gawo la alonda - zisanakhudze zotsatira zanu zowunikira.

Malingaliro Omaliza

Kuwonjezera wanuchromatography column moyo wautalisikungopulumutsa ndalama zokha, koma kusunga kukhulupirika kwa deta, kuchepetsa nthawi yopuma, ndi kupititsa patsogolo ntchito za labu. Ndi njira yoyenera yodzitetezera, mutha kuteteza chimodzi mwazinthu zamtengo wapatali za labu ndikuwonetsetsa kuti zotsatira zodalirika pakuthamanga kulikonse.

Mukufuna upangiri waukadaulo wamachitidwe achromatographic kapena kusankha kwazinthu?ContactChromasirlero-ife tiri pano kuti tikuthandizeni kuchita bwino kwa labu yanu ndi luntha laukadaulo komanso mayankho amunthu payekha.